cdx2 cre transgenic mice  (Jackson Laboratory)

Journal: Research Square

Article Title: An enteroendocrine-microbial axis in the large intestine controls host metabolism

doi: 10.21203/rs.3.rs-3112286/v1

Figure Lengend Snippet:

Article Snippet: These Neurog3 fl/fl mice were mated with animals containing the CDX2 Cre-ERT2 transgene and available from Jackson (JAX Strain #035169) .

Techniques: SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Cellular Physiology

Article Title: Growth differentiation factor 11 locally controls anterior–posterior patterning of the axial skeleton

doi: 10.1002/jcp.28904

Figure Lengend Snippet: Gdf11 expression is removed in posterior regions of Cdx2‐Cre ; Gdf11 flox/flox mice. (a) Whole‐mount in situ hybridization of mouse embryos at E9.5. Gdf11 expression patterns of wt and Cdx2‐Cre ; Gdf11 flox/flox embryos are shown. Dashed line with arrow heads indicates posterior regions that lack Gdf11 expression in a Cdx2‐Cre ; Gdf11 flox/flox embryo. (b) Cells expressing Cdx2‐Cre are marked by GFP expression in Cdx2‐Cre ; Gdf11 flox/+ ; Igs1 CKI‐mitoGFP/+ embryo at E9.5. (c) Newborn wt , Gdf11 −/− , and Cdx2‐Cre ; Gdf11 flox/flox pups. Both Gdf11 −/− and Cdx2‐Cre ; Gdf11 flox/flox mice display extended torso and truncated tails. (d) Area expressing Cdx2‐Cre is labeled by GFP expression in newborn Cdx2‐Cre ; Gdf11 flox/flox ; Igs1 CKI‐mitoGFP/+ mouse, and displayed laterally and ventrally. GFP, green fluorescent protein; wt, wild‐type [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: To analyze the effect of Cdx2‐Cre on Gdf11 flox/flox mice, Cdx2‐Cre transgenic male mice (Stock No. 009350), purchased from the Jackson Laboratory (Bar Harbor, ME), were first mated with Gdf11 flox/flox female mice.

Techniques: Expressing, In Situ Hybridization, Labeling

Journal: Journal of Cellular Physiology

Article Title: Growth differentiation factor 11 locally controls anterior–posterior patterning of the axial skeleton

doi: 10.1002/jcp.28904

Figure Lengend Snippet: Cdx2‐Cre ; Gdf11 flox/flox mice display abnormal skeletal patterning limited to posterior regions where Gdf11 expression is removed. (a–c) Alcian blue/Alizarin red staining of vertebral columns and vertebrosternal (true) ribs of newborn mice. True ribs attached to vertebral columns are shown in (c). Note that Cdx2‐Cre ; Gdf11 flox/flox mice exhibit extended lumbar observed in Gdf11 −/− mice but display normal true ribs. (d) Cells expressing Cdx2‐Cre are marked by GFP expression in newborn Cdx2‐Cre ; Gdf11 flox/flox ; Igs1 CKI‐mitoGFP/+ mouse. Red line points to the last (seventh) true rib. GFP, green fluorescent protein; wt, wild‐type [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: To analyze the effect of Cdx2‐Cre on Gdf11 flox/flox mice, Cdx2‐Cre transgenic male mice (Stock No. 009350), purchased from the Jackson Laboratory (Bar Harbor, ME), were first mated with Gdf11 flox/flox female mice.

Techniques: Expressing, Staining

Journal: Journal of Cellular Physiology

Article Title: Growth differentiation factor 11 locally controls anterior–posterior patterning of the axial skeleton

doi: 10.1002/jcp.28904

Figure Lengend Snippet: Skeletal analysis of wt , Gdf11 −/− , and Cdx2‐Cre; Gdf11 flox/flox mice

Article Snippet: To analyze the effect of Cdx2‐Cre on Gdf11 flox/flox mice, Cdx2‐Cre transgenic male mice (Stock No. 009350), purchased from the Jackson Laboratory (Bar Harbor, ME), were first mated with Gdf11 flox/flox female mice.

Techniques: Mutagenesis, Mouse Assay

Journal: Journal of Cellular Physiology

Article Title: Growth differentiation factor 11 locally controls anterior–posterior patterning of the axial skeleton

doi: 10.1002/jcp.28904

Figure Lengend Snippet: Craniofacial defects are observed in Gdf11 − / − mice, but not in Cdx2‐Cre ; Gdf11 flox/flox mice. (a) Representative micro‐CT images of newborn mouse skulls shown ventrally. Red arrows point to palatine bones, and yellow arrows indicate pterygoid processes. Note that cleft palate is observed only in Gdf11 −/ − mice. (b) Alcian blue/Alizarin red staining of newborn skulls. Boxed regions are shown at higher magnification. Yellow arrows indicate pterygoid processes. Cleft palate is observed in Gdf11 −/− mice, but not in Cdx2‐Cre ; Gdf11 flox/flox mice. micro‐CT, micro–computed tomography; wt, wild‐type [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: To analyze the effect of Cdx2‐Cre on Gdf11 flox/flox mice, Cdx2‐Cre transgenic male mice (Stock No. 009350), purchased from the Jackson Laboratory (Bar Harbor, ME), were first mated with Gdf11 flox/flox female mice.

Techniques: Micro-CT, Staining

Journal: Journal of Cellular Physiology

Article Title: Growth differentiation factor 11 locally controls anterior–posterior patterning of the axial skeleton

doi: 10.1002/jcp.28904

Figure Lengend Snippet: Schematic representation of vertebral columns indicating that locally expressed GDF11, not GDF11 secreted from the tail bud, controls axial skeletal patterning. Cdx2‐Cre ; Gdf11 flox/flox mice display an extended number of posterior vertebrae where GDF11 expression is removed, but normal patterning of anterior vertebrae. Cervical (orange)/thoracic (purple)/lumbar (sky blue) vertebrae, anterior tuberculi (small blue dots), sternums (red curves), and ribs (blue lines) are color‐coded as indicated. Gray‐dashed lines indicate normal vertebral positions: Six for the anterior tuberculum, 20 for the final thoracic vertebra, and 26 for the last lumbar vertebra. The green‐dashed line represents the upper limit of Cdx2‐Cre expression. GDF11, growth and differentiation factor 11; wt, wild‐type [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: To analyze the effect of Cdx2‐Cre on Gdf11 flox/flox mice, Cdx2‐Cre transgenic male mice (Stock No. 009350), purchased from the Jackson Laboratory (Bar Harbor, ME), were first mated with Gdf11 flox/flox female mice.

Techniques: Expressing